D7291
Deoxyribonuclease I RNase-free solution from bovine pancreas
Synonym(s):
DNase I, Deoxyribonuclease I, pancreatic DNase I, pancreatic deoxyribonuclease I
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About This Item
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biological source
bovine pancreas
grade
for molecular biology
form
buffered aqueous glycerol solution
specific activity
10000 units/mg protein
mol wt
29.1 kDa
concentration
5—15 mg protein/mL
technique(s)
DNA extraction: suitable
suitability
suitable for molecular biology
UniProt accession no.
application(s)
cell analysis
foreign activity
RNase, none detected
storage temp.
−20°C
Gene Information
cow ... DNASE1(282217)
General description
Deoxyribonuclease I digests single- and double-stranded DNA to a mixture of mono and oligonucleotides carrying 5′phosphates and 3′OH termini. This catalytic activity is divalent ion-dependent. In the presence of Mg2+, DNase I hydrolyzes each strand of double-stranded DNA randomly and independently. In the presence of Mn2+, both strands can be cleaved.
Application
Deoxyribonuclease I RNase-free solution from bovine pancreas has been used to digest DNA from various samples.
Used in molecular biology applications for removing DNA during RNA purification, for preparing DNA for nick translation, and for DNA-protein interaction analysis by footprinting methods.
Components
DNase I is provided in a solution of 50% (v/v) glycerol, 20 mM sodium acetate (pH 6.5), 5 mM CaCl2, and 0.1 mM PMSF.
Unit Definition
One unit will cause a ΔA260 of 0.001 per min per mL reaction mixture using calf thymus DNA as substrate.
Other Notes
RNA treated with this DNase I should not be used to generate a cDNA library or used for RT-PCR. For these sensitive applications, it is recommended to use DNase I, Amplification Grade , Catalog Number AMP-D1.
inhibitor
Product No.
Description
Pricing
related product
Product No.
Description
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Glutathione S-Transferase from E. coli, recombinant, expressed in E. coli, buffered aqueous solution
Ribonuclease A from bovine pancreas, for molecular biology, ≥70 Kunitz units/mg protein, lyophilized
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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