8505C

94090184, human thyroid, Epithelial

• UNSPSC Code: 41106514

Properties

• product name: 8505C, 94090184
• biological source: human thyroid
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human thyroid carcinoma, undifferentiated.

Cell Line Description

Established from undifferentiated thyroid carcinomas of a 78 year old female patient. Pathologically this primary carcinoma tissue contained residual well differentiated components suggesting well differentiated to undifferentiated carcinoma progression. Tumour suppresser genes p53, Rb, APC and MCC were analysed and sequence analysis confirmed a C:G to G:C transversion at the first base of p53 gene codon 248. Polymerase chain reaction-loss of heterozygosity assays confirmed allelic deletion of p53 gene. Loss of heterozygosity of tumour suppresser genes was not observed.

Application

8505C cell line has been used to study the impact of synthetic manipulation of expression of microRNAs miR-25 and miR-222 in benign and malignant thyroid cells. It has also been used to determine the induction of pluripotency markers by mitogen activated protein kinase (MEK) inhibition.

Culture Medium

EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Doubling time is 36 hours. Saturation density at confluency is 1x100,000 cells/cm².

Other Notes

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