03-0190-00
SMC® Human IL-18 High Sensitivity Immunoassay Kit
Synonym(s):
IGIF immunoassay kit, IL-18 Single Molecule Counting Kit, IL-18 ultra-sensitive immunoassay, Interleukin-18 immunoassay kit
About This Item
Recommended Products
species reactivity
mouse, feline, rat
manufacturer/tradename
SMC®
assay range
sensitivity: 0.09 pg/mL
(Lower Limit of Quantification)
inter-assay cv: 7%
intra-assay cv: 4%
(five normal plasma samples run in triplicate by 3 different operators on 3 different days.)
technique(s)
single molecule counting: suitable
storage temp.
2-8°C
Related Categories
General description
Assay protocols are similar to existing sandwich ELISA methods with two key differences:
1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification.
2) The Erenna and SMCxPRO® immunoassay systems detect analytes using Single Molecule Counting (SMC®) technology.
Specificity
Specificity to the following species recombinant proteins were tested with the following results:
Feline – 0.5% Mouse – 4% Rat – 0.3%
Cross Reactivity:
No cross-reactivity was seen with the following cytokines: IL-1a, IL-1ß, IL-1RA, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IL-17E, IL-7F, IL-22, IL-27.
Accuracy:
- Minimum of 5 serum samples spiked with average recovery of 99% with a range of 92-107%
- Minimum of 5 plasma samples spiked with average recovery of 95% with a range of 88-103%
Application
This new streamline kit has the following features including:
- Improved options for sample prep and incubation
- Lyophilization of the standard provides the kit at one storage condition
- Increased reagent volumes to accommodate automation
- Validated to run on both SMCxPRO® and Erenna
Research Category:
Inflammation & Immunology
Research Sub-Category:
- Inflammatory Disease,
- Immune Response
- Autoimmune disease
Packaging
Components
- Assay Buffer
- IL-18 Coated Beads
- Standard Diluent
- IL-18 Detection Antibody
- IL-18 Standard
- 10X Wash Buffer
- Buffer D
- Elution Buffer B
Legal Information
Disclaimer
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B
Storage Class Code
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
Certificates of Analysis (COA)
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