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LC/MS Analysis of Erythromycin Analogs on Ascentis® Express HILIC

LC/MS Analysis of Erythromycin Analogs on Ascentis® Express HILIC application for HPLC

Matériaux

Colonne analytique

Réf. du produit
Description
Tarif

53981-U

Ascentis® Express HILIC HPLC Column

2.7 μm particle size, L × I.D. 15 cm × 4.6 mm

Composant de phase mobile

Réf. du produit
Description
Tarif

70221

Formate d′ammonium

eluent additive for LC-MS, LiChropur™, ≥99.0%

Étalon

Réf. du produit
Description
Tarif

E8755

Erythromycin ethyl succinate

45674

Erythromycin

tested according to Ph. Eur.

1242021

Erythromycin C

United States Pharmacopeia (USP) Reference Standard

CONDITIONS

column

Ascentis Express HILlC, 15 cm x 4.6 mm I.D., 2.7 μm particles (53981-U)

mobile phase

[A] 100 mM ammonium formate, pH 3.0 (titrated with formic acid); [B] acetonitrile; (10:90, A:B)

flow rate

1.0 mL/min

pressure

1247 psi (86 bar)

column temp.

35 °C

detector

ESI(+); SIR

injection

5 μL

Description

Remarque sur l'analyse

This application shows the suitability of Ascentis Express Silica columns for the analysis of erythromycin analogs in aqueous normal phase/HILIC mode of chromatography. Other relatively hydrophobic aminoglycosides have also been shown to separate under these conditions.

Informations légales

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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