- Chemically-activatable alkyne-tagged probe for imaging microdomains in lipid bilayer membranes.
Chemically-activatable alkyne-tagged probe for imaging microdomains in lipid bilayer membranes.
Scientific reports (2017-01-25)
Satoshi Yamaguchi, Taku Matsushita, Shin Izuta, Sumika Katada, Manami Ura, Taro Ikeda, Gosuke Hayashi, Yuta Suzuki, Koya Kobayashi, Kyoya Tokunaga, Yasuyuki Ozeki, Akimitsu Okamoto
PMID28117375
ABSTRACT
A chemically-activatable alkynyl steroid analogue probe has been synthesized for visualizing the lipid raft membrane domains by Raman microscopy. The Raman probe, in which ring A of its steroid backbone is replaced with an alkynyl group, was designed to enable activation of the alkyne signal through the Eschenmoser-Tanabe fragmentation reaction of the oxidized cholesterol precursor in lipid bilayer membranes. The alkynyl steroid analogue was observed to form liquid-ordered raft-like domains on a model giant-liposome system in a similar manner as cholesterol, and the large alkyne signal of the accumulated probe at 2120 cm
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