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  • In vivo transcriptional regulation of N-Myc target genes is controlled by E-box methylation.

In vivo transcriptional regulation of N-Myc target genes is controlled by E-box methylation.

Proceedings of the National Academy of Sciences of the United States of America (2005-08-12)
Giovanni Perini, Daniel Diolaiti, Antonio Porro, Giuliano Della Valle
ABSTRACT

N-Myc is a transcription factor that forms heterodimers with the protein Max and binds gene promoters by recognizing a DNA sequence, CACGTG, called E-box. The identification of N-myc target genes is an important step for understanding N-Myc biological functions in both physiological and pathological contexts. In this study, we describe the identification of N-Myc-responsive genes through chromatin immunoprecipitation and methylation-sensitive restriction analysis. Results show that N-Myc is a direct regulator of several identified genes, and that methylation of the CpG dinucleotide within the E-box prevents the access of N-Myc to gene promoters in vivo. Furthermore, methylation profile of the E-box within the promoters of EGFR and CASP8, two genes directly controlled by Myc, is cell type-specific, suggesting that differential E-box methylation may contribute to generating unique patterns of Myc-dependent transcription. This study illuminates a central role of DNA methylation in controlling N-Myc occupancy at gene promoters and modulating its transcriptional activity in cancer cells.

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Anti-Actin antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
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Anti-N-Myc Mouse mAb (NCM II 100), liquid, clone NCM II 100, Calbiochem®
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