Skip to Content
Merck
  • Screening and Confirmatory Analyses of Flunixin in Tissues and Bodily Fluids after Intravenous or Intramuscular Administration to Cull Dairy Cows with or without Lipopolysaccharide Challenge.

Screening and Confirmatory Analyses of Flunixin in Tissues and Bodily Fluids after Intravenous or Intramuscular Administration to Cull Dairy Cows with or without Lipopolysaccharide Challenge.

Journal of agricultural and food chemistry (2015-12-24)
Weilin L Shelver, David J Smith, Lisa A Tell, Ronald E Baynes, J W Schroeder, Jim E Riviere
ABSTRACT

Twenty cull dairy cows (645 ± 83 kg) were treated with 2.2 mg/kg bw flunixin by intravenous (IV) or intramuscular (IM) administration with, or without, exposure to lipopolysaccharide in a two factor balanced design. The usefulness of screening assays to identify violative flunixin levels in a variety of easily accessible ante-mortem fluids in cattle was explored. Two animals with violative flunixin liver residue and/or violative 5-hydroxy flunixin milk residues were correctly identified by a flunixin liver ELISA screen. Oral fluid did not produce anticipated flunixin concentration profiles using ELISA determination. One cow that had liver and milk violative residues, and one cow that had a milk violation at the prescribed withdrawal period were correctly identified by flunixin milk lateral flow analyses. The ratio of urinary flunixin and 5-hydroxy flunixin may be useful for predicting disruption of metabolism caused by disease or other factors potentially leading to violative liver flunixin residues.

MATERIALS
Product Number
Brand
Product Description

Supelco
Flunixin-d3, VETRANAL®, analytical standard
SKU
Pack Size
Availability
Price
Quantity
Supelco
5-Hydroxyflunixin, VETRANAL®, analytical standard
SKU
Pack Size
Availability
Price
Quantity
Supelco
Flunixin, VETRANAL®, analytical standard
SKU
Pack Size
Availability
Price
Quantity