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  • Incorporation of mouse APOBEC3 into murine leukemia virus virions decreases the activity and fidelity of reverse transcriptase.

Incorporation of mouse APOBEC3 into murine leukemia virus virions decreases the activity and fidelity of reverse transcriptase.

Journal of virology (2014-04-11)
Stefano Boi, Angelo Kolokithas, Joyce Shepard, Rebecca Linwood, Kyle Rosenke, Erik Van Dis, Frank Malik, Leonard H Evans
ABSTRACT

APOBEC3 proteins are restriction factors that induce G→A hypermutation in retroviruses during replication as a result of cytidine deamination of minus-strand DNA transcripts. However, the mechanism of APOBEC inhibition of murine leukemia viruses (MuLVs) does not appear to be G→A hypermutation and is unclear. In this report, the incorporation of mA3 in virions resulted in a loss in virion reverse transcriptase (RT) activity and RT fidelity that correlated with the loss of virion-specific infectivity.

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Roche
Reverse Transcriptase Assay, colorimetric, suitable for enzyme immunoassay, kit of 1 (14 components), sufficient for ≤200 tests
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