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  • IL-27 improves migrational and antiviral potential of CB dendritic cells.

IL-27 improves migrational and antiviral potential of CB dendritic cells.

Human immunology (2014-02-18)
Julia Birkholz, Aysefa Doganci, Claudia Darstein, Stephan Gehring, Fred Zepp, Claudius U Meyer
ABSTRACT

Interleukin (IL)-27 is known to be increased considerably in cord blood (CB) dendritic cells (DCs) after TLR ligation. Previously, we demonstrated that also basal IL-27 levels are higher in CB DCs. Here, we examined effects of IL-27 on monocyte derived dendritic cells (moDCs) to approach its particular role in the specialized immune system of the human neonate. Exogenous IL-27 promotes IL-27 transcription in CB and adult blood (AB) moDCs. IL-27 acts on CB moDCs primarily by significantly augmenting IL-27 protein, secondarily by increasing transcription of CXCL10 among other chemokines, chemokine receptor CCR1, interferon stimulated genes, transcription factor IRF8 and genes involved in antigen presentation. Furthermore, CB moDCs respond to IL-27 with augmented IL-8 and Tumor necrosis factor (TNF)-α. The results suggest that IL-27 enhances migrational and antiviral properties of CB dendritic cells.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Mouse CRG-2 / CXCL10 ELISA Kit, for serum, plasma and cell culture supernatant
Pricing and availability is not currently available.
Sigma-Aldrich
Human IP-10 / CXCL10 ELISA Kit, for serum, plasma, cell culture supernatant and urine
Pricing and availability is not currently available.