Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast.

Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast.

Gene (2014-02-04)

Gonensin O Bozdag, Alaattin Kaya, Ahmet Koc, Gundula A Noll, Dirk Prüfer, Hüseyin Caglar Karakaya

ABSTRACT

Nickel is an essential micronutrient due to its involvement in many enzymatic reactions as a cofactor. However, excess of this element is toxic to biological systems. Here, we constructed a cDNA library from Beta maritima and screened it in the yeast system to identify genes that confer resistance to toxic levels of nickel. A cDNA clone (NIC6), which encodes for a putative membrane protein with unknown function, was found to help yeast cells to tolerate toxic levels of nickel. A GFP fused form of Nic6 protein was localized to multivesicular structures in tobacco epidermal cells. Thus, our results suggest a possible role of Nic6 in nickel and intracellular ion homeostasis.

MATERIALS

Product Number

Brand

Product Description

267074

Sigma-Aldrich

Nickel, rod, diam. 6.35 mm, ≥99.99% trace metals basis

266981

Sigma-Aldrich

Nickel, powder, <50 μm, 99.7% trace metals basis

357553

Sigma-Aldrich

Nickel, foil, thickness 0.5 mm, 99.98% trace metals basis

357626

Sigma-Aldrich

Nickel, wire, diam. 0.5 mm, ≥99.9% trace metals basis

577995

Sigma-Aldrich

Nickel, nanopowder, <100 nm avg. part. size, ≥99% trace metals basis

357588

Sigma-Aldrich

Nickel, foil, thickness 0.1 mm, 99.98% trace metals basis

GF38428241

Nickel, foil, 25mm disks, thickness 0.025mm, annealed, 99%

GF85965250

Nickel, tube, 100mm, outside diameter 3.0mm, inside diameter 2.4mm, wall thickness 0.3mm, hard, 99.5%

GF60907091

Nickel, wire reel, 20m, diameter 1.0mm, hard, 99.98%

GF57875417

Nickel, tube, 200mm, outside diameter 5.9mm, inside diameter 5.65mm, wall thickness 0.125mm, hard, 99.5%

GF23066197

Nickel, tube, 200mm, outside diameter 0.8mm, inside diameter 0.64mm, wall thickness 0.08mm, as drawn, 99.5%

GF64460099

Nickel, tube, 1000mm, outside diameter 1.8mm, inside diameter 1.6mm, wall thickness 0.1mm, hard, 99.5%

GF46969587

Nickel, wire reel, 5m, diameter 0.5mm, as drawn, 99%

GF47594144

Nickel, tube, 1000mm, outside diameter 0.8mm, inside diameter 0.3mm, wall thickness 0.25mm, as drawn, 99.5%

GF42115115

Nickel, foil, 15mm disks, thickness 0.5mm, as rolled, 99.99+%

GF63069419

Nickel, foil, 25mm disks, thickness 0.15mm, annealed, 99%

GF38850576

Nickel, wire reel, 50m, diameter 0.025mm, as drawn, 99.6+%

GF29666920

Nickel, foil, 20m coil, thickness 0.125mm, coil width 1mm, as rolled, 99%

GF93376941

Nickel, tube, 100mm, outside diameter 0.75mm, inside diameter 0.59mm, wall thickness 0.08mm, hard, 99.5%

GF43517976

Nickel, wire reel, 20m, diameter 2.0mm, hard, 99.98%

GF05191578

Nickel, foil, 15mm disks, thickness 0.20mm, annealed, 99%

GF44029425

Nickel, foil, 1m coil, thickness 0.15mm, annealed, 99%

GF04942414

Nickel, wire reel, 5m, diameter 0.5mm, hard, 99.98%

GF74554780

Nickel, wire reel, 50m, diameter 0.5mm, as drawn, 99%

GF61415030

Nickel, tube, 200mm, outside diameter 2.4mm, inside diameter 2.248mm, wall thickness 0.076mm, hard, 99.5%

GF39981437

Nickel, wire reel, 5m, diameter 0.25mm, as drawn, 99.99+%

GF50019937

Nickel, foil, 6mm disks, thickness 0.009mm, 99.95%

GF84292364

Nickel, wire reel, 2m, diameter 0.75mm, as drawn, 99.99+%

GF01966732

Nickel, wire reel, 5m, diameter 1.0mm, as drawn, 99%

GF57335148

Nickel, wire reel, 50m, diameter 0.5mm, hard, 99.98%