Germination and root elongation bioassays in six different plant species for testing Ni contamination in soil.

Germination and root elongation bioassays in six different plant species for testing Ni contamination in soil.

Bulletin of environmental contamination and toxicology (2013-11-30)

Giovanna Visioli, Federica D Conti, Ciro Gardi, Cristina Menta

ABSTRACT

In vitro short-term chronic phytotoxicity germination and root elongation test were applied to test the effects of nickel (Ni) in seed germination and root elongation in six plants species: Cucumis sativus (Cucurbitaceae), Lepidium sativum and Brassica nigra (Brassicaceae), Trifolium alexandrinum and Medicago sativa (Fabaceae), Phacelia tanacetifolia (Boraginaceae). A naturally Ni rich soil was used to compare the results obtained. Unlike root elongation, germination was not affected by Ni in any of the six species tested. EC50 values, calculated on the root elongation, showed that Ni toxicity decreases in the following order: P. tanacetifolia > B. nigra > C. sativus > L. sativum > M. sativa > T. alexandrinum. The test conducted using soil elutriate revealed a significantly lower effect in both seed germination and root elongation when compared to the results obtained using untreated soil. Conversely, the test performed on soil confirmed the high sensitivity of C. sativus, P. tanacetifolia and L. sativum to Ni.

MATERIALS

Product Number

Brand

Product Description

267074

Sigma-Aldrich

Nickel, rod, diam. 6.35 mm, ≥99.99% trace metals basis

266981

Sigma-Aldrich

Nickel, powder, <50 μm, 99.7% trace metals basis

357553

Sigma-Aldrich

Nickel, foil, thickness 0.5 mm, 99.98% trace metals basis

357626

Sigma-Aldrich

Nickel, wire, diam. 0.5 mm, ≥99.9% trace metals basis

577995

Sigma-Aldrich

Nickel, nanopowder, <100 nm avg. part. size, ≥99% trace metals basis

357588

Sigma-Aldrich

Nickel, foil, thickness 0.1 mm, 99.98% trace metals basis

GF38428241

Nickel, foil, 25mm disks, thickness 0.025mm, annealed, 99%

GF85965250

Nickel, tube, 100mm, outside diameter 3.0mm, inside diameter 2.4mm, wall thickness 0.3mm, hard, 99.5%

GF60907091

Nickel, wire reel, 20m, diameter 1.0mm, hard, 99.98%

GF57875417

Nickel, tube, 200mm, outside diameter 5.9mm, inside diameter 5.65mm, wall thickness 0.125mm, hard, 99.5%

GF23066197

Nickel, tube, 200mm, outside diameter 0.8mm, inside diameter 0.64mm, wall thickness 0.08mm, as drawn, 99.5%

GF64460099

Nickel, tube, 1000mm, outside diameter 1.8mm, inside diameter 1.6mm, wall thickness 0.1mm, hard, 99.5%

GF46969587

Nickel, wire reel, 5m, diameter 0.5mm, as drawn, 99%

GF47594144

Nickel, tube, 1000mm, outside diameter 0.8mm, inside diameter 0.3mm, wall thickness 0.25mm, as drawn, 99.5%

GF42115115

Nickel, foil, 15mm disks, thickness 0.5mm, as rolled, 99.99+%

GF63069419

Nickel, foil, 25mm disks, thickness 0.15mm, annealed, 99%

GF38850576

Nickel, wire reel, 50m, diameter 0.025mm, as drawn, 99.6+%

GF29666920

Nickel, foil, 20m coil, thickness 0.125mm, coil width 1mm, as rolled, 99%

GF93376941

Nickel, tube, 100mm, outside diameter 0.75mm, inside diameter 0.59mm, wall thickness 0.08mm, hard, 99.5%

GF43517976

Nickel, wire reel, 20m, diameter 2.0mm, hard, 99.98%

GF05191578

Nickel, foil, 15mm disks, thickness 0.20mm, annealed, 99%

GF44029425

Nickel, foil, 1m coil, thickness 0.15mm, annealed, 99%

GF04942414

Nickel, wire reel, 5m, diameter 0.5mm, hard, 99.98%

GF74554780

Nickel, wire reel, 50m, diameter 0.5mm, as drawn, 99%

GF61415030

Nickel, tube, 200mm, outside diameter 2.4mm, inside diameter 2.248mm, wall thickness 0.076mm, hard, 99.5%

GF39981437

Nickel, wire reel, 5m, diameter 0.25mm, as drawn, 99.99+%

GF50019937

Nickel, foil, 6mm disks, thickness 0.009mm, 99.95%

GF84292364

Nickel, wire reel, 2m, diameter 0.75mm, as drawn, 99.99+%

GF01966732

Nickel, wire reel, 5m, diameter 1.0mm, as drawn, 99%

GF57335148

Nickel, wire reel, 50m, diameter 0.5mm, hard, 99.98%