Skip to Content
Merck
  • Quantitative aspects of the Naphthol Yellow S staining for proteins studied in a model system of polyacrylamide films and in isolated rat liver cells and nuclei.

Quantitative aspects of the Naphthol Yellow S staining for proteins studied in a model system of polyacrylamide films and in isolated rat liver cells and nuclei.

Acta histochemica. Supplementband (1979-01-01)
J Tas, M van der Ploeg, J James
PMID95604
ABSTRACT

After staining with Naphthol Yellow S (NYS) at optimal conditions of pH (2.8), the protein content of rat liver cells isolated by means of a collagenase perfusion technique was found to be cytophotometrically immeasurable, because of too high local dye absorbances. In order to lower the absorption values, techniques to flatten the cells, off-peak measurements and NYS staining at non-optimal pH levels were applied. With polyacrylamide model films incorporated with albumin, the reliability of off-peak measurements and the quantitative aspects of the modified protein staining procedures have been investigated. It was found that NYS is a quantitative protein stain not only at pH 2.8, but also at pH 2.0, 3.5 and 4.0 respectively. Off-peak measurements can also produce quantitative results. In cases of a combined Feulgen-NYS staining, the Feulgen-DNA values were not significantly influenced by any of the NYS staining procedures.

MATERIALS
Product Number
Brand
Product Description

Supelco
Naphthol Yellow S, analytical standard
Pricing and availability is not currently available.
Sigma-Aldrich
Naphthol Yellow S, for microscopy (Hist.), for the precipitation (of amino acids and peptides)
Pricing and availability is not currently available.