- Impact of sorbic acid on germination and outgrowth heterogeneity of Bacillus cereus ATCC 14579 spores.
Impact of sorbic acid on germination and outgrowth heterogeneity of Bacillus cereus ATCC 14579 spores.
Applied and environmental microbiology (2012-09-25)
Heidy M W den Besten, Clint C J van Melis, Jan Willem Sanders, Masja N Nierop Groot, Tjakko Abee
PMID23001664
ABSTRACT
Population heterogeneity complicates the predictability of the outgrowth kinetics of individual spores. Flow cytometry sorting and monitoring of the germination and outgrowth of single dormant spores allowed the quantification of acid-induced spore population heterogeneity at pH 5.5 and in the presence of sorbic acid. This showed that germination efficiency was not a good predictor for heterogeneity in final outgrowth.