Skip to Content
Merck
  • Eimeria tenella: effects of diclazuril treatment on microneme genes expression in second-generation merozoites and pathological changes of caeca in parasitized chickens.

Eimeria tenella: effects of diclazuril treatment on microneme genes expression in second-generation merozoites and pathological changes of caeca in parasitized chickens.

Experimental parasitology (2010-02-09)
Bian-hua Zhou, Hong-wei Wang, Xiao-yang Wang, Li-fang Zhang, Ke-yu Zhang, Fei-qun Xue
ABSTRACT

The effects of diclazuril on mRNA expression levels of invasion-related microneme genes were examined in second-generation merozoites of Eimeria tenella (E. tenella) by quantitative real-time (QRT) PCR. Diclazruil treatment of infected chickens significantly decreased the number of second-generation merozoites by 65.13%, and resulted in downregulation of EtMIC genes: EtMIC1 by 65.63%, EtMIC2 by 64.12%, EtMIC3 by 56.82%, EtMIC4 by 73.48%, and EtMIC5 by 78.17%. SEM images of caecum tissue from uninfected chickens showed regular intestinal villus structure. In infected chickens, a distinct loss of the superficial epithelium, with a flattened mucosa and large-area necrosis and anabrosis, was evident. In diclazruil-treated chickens, a decrease in merozoite number and a visibly improved appearance of the caeca were noted. These improvements appeared to be mediated in part by downregulation of the expression of invasion-related EtMIC genes in response to diclazuril.

MATERIALS
Product Number
Brand
Product Description

Diclazuril for system suitability, European Pharmacopoeia (EP) Reference Standard
Pricing and availability is not currently available.
Supelco
Diclazuril, VETRANAL®, analytical standard
Pricing and availability is not currently available.