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  • Cavin4 interacts with Bin1 to promote T-tubule formation and stability in developing skeletal muscle.

Cavin4 interacts with Bin1 to promote T-tubule formation and stability in developing skeletal muscle.

The Journal of cell biology (2021-10-12)
Harriet P Lo, Ye-Wheen Lim, Zherui Xiong, Nick Martel, Charles Ferguson, Nicholas Ariotti, Jean Giacomotto, James Rae, Matthias Floetenmeyer, Shayli Varasteh Moradi, Ya Gao, Vikas A Tillu, Di Xia, Huang Wang, Samira Rahnama, Susan J Nixon, Michele Bastiani, Ryan D Day, Kelly A Smith, Nathan J Palpant, Wayne A Johnston, Kirill Alexandrov, Brett M Collins, Thomas E Hall, Robert G Parton
ABSTRACT

The cavin proteins are essential for caveola biogenesis and function. Here, we identify a role for the muscle-specific component, Cavin4, in skeletal muscle T-tubule development by analyzing two vertebrate systems, mouse and zebrafish. In both models, Cavin4 localized to T-tubules, and loss of Cavin4 resulted in aberrant T-tubule maturation. In zebrafish, which possess duplicated cavin4 paralogs, Cavin4b was shown to directly interact with the T-tubule-associated BAR domain protein Bin1. Loss of both Cavin4a and Cavin4b caused aberrant accumulation of interconnected caveolae within the T-tubules, a fragmented T-tubule network enriched in Caveolin-3, and an impaired Ca2+ response upon mechanical stimulation. We propose a role for Cavin4 in remodeling the T-tubule membrane early in development by recycling caveolar components from the T-tubule to the sarcolemma. This generates a stable T-tubule domain lacking caveolae that is essential for T-tubule function.

MATERIALS
Product Number
Brand
Product Description

Roche
Anti-Digoxigenin-AP, Fab fragments, from sheep
Pricing and availability is not currently available.
Sigma-Aldrich
Anti-Bin1, clone 99D (Ascites Free) Antibody, clone 99D, 1 mg/mL, from mouse
Pricing and availability is not currently available.
Millipore
Protease Inhibitor Cocktail Set III, EDTA-Free, Protease inhibitor cocktail III, EDTA-free for inhibiting aspartic, cysteine, and serine proteases as well as aminopeptidases in mammalian cells and tissues.
Pricing and availability is not currently available.
Sigma-Aldrich
Monoclonal Anti-Actin (α-Sarcomeric) antibody produced in mouse, clone 5C5, ascites fluid
Pricing and availability is not currently available.
Sigma-Aldrich
DAPI, for nucleic acid staining
Pricing and availability is not currently available.