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  • Drought Stress Causes Specific Changes to the Spliceosome and Stress Granule Components.

Drought Stress Causes Specific Changes to the Spliceosome and Stress Granule Components.

Frontiers in molecular biosciences (2020-02-11)
Claudius Marondedze, Ludivine Thomas, Kathryn S Lilley, Chris Gehring
ABSTRACT

The spliceosome processes RNAs from a pre-RNA state to a mature mRNA thereby influencing RNA availability for translation, localization, and turnover. It consists of complex structures containing RNA-binding proteins (RBPs) essential for post-transcriptional gene expression control. Here we investigate the dynamic modifications of spliceosomal RBPs under stress and in particular drought stress. We do so by mRNA interactome capture in Arabidopsis thaliana using label free quantitation. This approach identified 44 proteins associated with the spliceosome and further 32 proteins associated with stress granules. We noted a high enrichment in the motifs RDRR and RSRSRS that are characteristic of RNA interacting proteins. Identification of splicing factors reflect direct and/or indirect stress induced splicing events that have a direct effect on transcriptome and proteome changes under stress. Furthermore, detection of stress granule components is consistent with transcriptional arrest. Identification of drought induced stress granule components is critical in determining common abiotic stress-induced foci that can have biotechnological applications. This study may therefore open ways to modify plant stress responses at a systems level through the modification of key spliceosome components.

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L-Rhamnose, natural sourced, 99%, FG
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100 μL
1 Estimated to ship on February 18, 2025
CHF 417.00

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