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  • Scrutinizing calcium flux oscillations in T lymphocytes to deduce the strength of stimulus.

Scrutinizing calcium flux oscillations in T lymphocytes to deduce the strength of stimulus.

Scientific reports (2015-01-15)
Susan N Christo, Kerrilyn R Diener, Robert E Nordon, Michael P Brown, Hans J Griesser, Krasimir Vasilev, Farid C Christo, John D Hayball
PMID25585590
ABSTRACT

The capture and activation of individual T cells on functionalised surfaces enables real-time analyses of the magnitude and rhythm of intracellular calcium release. Application of Haarlet transformations generate a calcium flux 'threshold', with the frequency of the 'threshold crossings' correlating with the strength of the original T cell stimulus. These findings represent a new method to evaluate graduations in T cell activation in real time, and at a single-cell level.

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