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Key Documents

SRP8039

Sigma-Aldrich

Irisin

recombinant, expressed in CHO cells, FLAG® tagged, >95% (SDS-PAGE)

Synonym(s):

CD121a, Fibronectin type III domain-containing protein 5 (cleaved), Fibronectin type III repeat-containing protein 2 (cleaved)

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About This Item

CAS Number:
1465928-51-1
UNSPSC Code:
12352202
NACRES:
NA.32

recombinant

expressed in CHO cells

tag

FLAG® tagged

Assay

>95% (SDS-PAGE)

form

lyophilized

mol wt

~26 kDa

packaging

pkg of 10 μg

impurities

<0.1 EU/μg endotoxin, tested

UniProt accession no.

Q8NAU1

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... FNDC5(252995)

General description

Irisin is an described exercise-induced hormone secreted by skeletal muscle in mice and humans. Irisin is cleaved from the type I membrane protein FNDC5 (fibronectin type III domain containing 5). FNDC5 is mainly expressed in the muscles. Low levels of the mRNA are also present in the kidney, liver and lung. The gene encoding irisin is mapped to human chromosome 1p35.1.

Biochem/physiol Actions

Irisin activates beige fat cells (beige cells have a gene expression pattern distinct from either white or brown fat and are preferentially sensitive to the polypeptide hormone Irisin) and improves systemic metabolism by increasing energy expenditure. It is mainly responsible for the conversion of white fat into brown fat. This conversion enhances metabolic uncoupling and caloric expenditure. Irisin is always associated with beneficial responses of exercise on cellular metabolism. In obese and type 2 diabetic patients, low levels of circulating irisin are characteristic of the adipose tissue and muscle.

Physical form

Lyophilized from PBS.

Reconstitution

Reconstitute with 100 μL deionized water.

Other Notes

Irisin (human, aa 16-127 /mouse, aa 29-140) is Flag-tagged at the N-terminus. Irisin has 100% identity betwen mouse, rat, monkey and human.

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Common genetic variation in the human FNDC5 locus, encoding the novel muscle-derived 'browning' factor irisin, determines insulin sensitivity.
Staiger H, et al.
PLoS ONE, 8, e61903-e61903 (2013)
FNDC5 and irisin in humans: I. Predictors of circulating concentrations in serum and plasma and II. mRNA expression and circulating concentrations in response to weight loss and exercise.
Huh JY, et al.
Metabolism, Clinical and Experimental, 61, 1725-1725 (2012)
Is irisin a human exercise gene?
Timmons JA
Nature, 488, E9-10 (2012)
Irisin is expressed and produced by human muscle and adipose tissue in association with obesity and insulin resistance.
Moreno-Navarrete JM, et al.
The Journal of Clinical Endocrinology and Metabolism, 98, E769-E778 (2013)

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