SAB4200880

Anti-Cellular Fibronectin antibody, Mouse monoclonal

clone FN-3E2, purified from hybridoma cell culture

• Synonym(s): cFN
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• antibody form: purified from hybridoma cell culture
• Quality Level: 200
• clone: FN-3E2
• form: liquid
• species reactivity: mouse, rat, human, monkey, chicken
• concentration: ~1 mg/mL
• isotype: IgM
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Fibronectin is an extracellular matrix protein composed of two nearly identical disulfide-bound polypeptides with typical molecular weights of 220 - 240 kDa.1 Cellular fibronectin is structurally and antigenically similar to cold insoluble globulin from plasma.

Specificity

Monoclonal Anti-Cellular Fibronectin labels fibronectin in the pericellular extracellular matrix of cultured chicken fibroblasts. It also labels cellular fibronectin in chicken embryo, mouse and human fibroblasts grown in tissue culture using in indirect immunofluorescent labeling technique.

Application

The antibody may also be used in immunoperoxidase or immunofluorescent labeling on frozen human tissue preparations. In immunoblotting, Monoclonal Anti-Cellular Fibronectin localizes the 240 kDa bands of cellular fibronectin in both its native and denatured forms.

Biochem/physiol Actions

Analysis of the fibronectin molecule indicates that it contains several functionally and structurally distinct domains, which may bind to cell surfaces, collagen, heparin, etc.2,3,4,5. Numerous studies have shown the fibronectin may enhance cell adhesion and spreading and affect the routes of cell migration both in vivo and in culture.6 It has been shown that upon malignant transformation many cells lose their surface bound fibronectin.7

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. Protect from prolonged exposure to light.

Disclaimer

This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1