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MAK115

Sigma-Aldrich

Hemoglobin Assay Kit

sufficient for 250 colorimetric tests

Synonym(s):

Hemoglobin Quantitation Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 250 colorimetric tests

detection method

colorimetric

relevant disease(s)

hematological disorder

storage temp.

2-8°C

Gene Information

human ... HBA1(3039) , HBA2(3040) , HBB(3043)
mouse ... HBB(15129)
rat ... HBB(24440)

General description

Hemoglobin (Hb) is an iron-containing metalloprotein that serves as the primary means of oxygen transport in vertebrates. Hemoglobin is primarily found in red blood cells where it makes up to 97% of the cell′s dry content. Hemoglobin can also be found in other tissues where it serves as an antioxidant. Alterations in blood hemoglobin levels occurs in many diseases such as anemia and polycytheima.

Application

Hemoglobin Assay Kit has been used to measure the concentration of hemoglobin.

Features and Benefits

Compatible with high-throughput handling systems. Can be adapted for use with cuvettes.

Suitability

Suitable for the detection of hemoglobinin a variety of samples such as blood, serum, plasma, and urine. This kit can be used to study the effects of various compounds on hemoglobin metabolism and levels.

Principle

This assay is based on the improved Triton™[NP1]/NaOH method in which hemoglobin is converted to a colorimetric product measured at 400 nm. This assay has a linear detection range between 0.9-200 mg/dL in the 96 well plate assay.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Pictograms

CorrosionEnvironment
GHS05,GHS09

Signal Word

Warning

Hazard Statements

H290,H319,H410

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Irrit. 2 - Met. Corr. 1

Storage Class Code

8B - Non-combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Polycythemia vera masked due to severe iron deficiency anemia.
Kambali S and Asma T
Hematology/oncology and Stem Cell Therapy (2016)
M J Faulkner et al.
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Eighteen multiparous cows were used in a split-plot replicated Latin square with two 28-d periods to evaluate the effects of source of supplemental Cu, Zn, and Mn (sulfates or hydroxy) on apparent absorption of minerals when fed in either a
Malthe Hvas et al.
Conservation physiology, 5(1), cox066-cox066 (2017-12-09)
The parasitic amoeba Paramoeba perurans is an aetiological agent of amoebic gill disease (AGD), a serious problem in seawater salmonid aquaculture globally. Other finfish species are also infected and infection events may be associated with periods of unusual high temperatures.
Biochemical markers of oxidative stress in Saudi women with recurrent miscarriage.
Ghneim H K and Mashael M A
Journal of Korean Medical Science, 31(1), 98-105 (2016)
Cheng Chen et al.
Cell death & disease, 11(2), 135-135 (2020-02-23)
Diamond-Blackfan anemia (DBA) is a rare, inherited bone marrow failure syndrome, characterized by red blood cell aplasia, developmental abnormalities, and enhanced risk of malignancy. However, the underlying pathogenesis of DBA is yet to be understood. Recently, mutations in the gene
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