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C5670

Sigma-Aldrich

Calcium chloride

anhydrous, BioReagent, suitable for insect cell culture, suitable for plant cell culture, ≥96.0%

Synonym(s):

CaCl2, Calcium dichloride

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About This Item

Linear Formula:
CaCl2
CAS Number:
10043-52-4
Molecular Weight:
110.98
EC Number:
233-140-8
MDL number:
MFCD00010903
UNSPSC Code:
12352207
PubChem Substance ID:
24892577
NACRES:
NA.71

grade

anhydrous

vapor pressure

0.01 mmHg ( 20 °C)

product line

BioReagent

Assay

≥96.0%

form

powder

technique(s)

cell culture | insect: suitable
cell culture | plant: suitable

mp

772 °C (lit.)

SMILES string

[Cl-].[Cl-].[Ca++]

InChI

1S/Ca.2ClH/h;2*1H/q+2;;/p-2

InChI key

UXVMQQNJUSDDNG-UHFFFAOYSA-L

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General description

Calcium chloride is a commonly used reagent in biochemistry. Calcium plays important roles in many biological processes, including signal transduction, muscle contraction, and maintenance of cell membrane and cell wall stability.1,2 Extensive reviews of the experimental measurement of biological calcium have been published.3,4Calcium chloride is used in the preparation and transformation of competent E. coli and in the transfection of eukaryotic cells with either plasmid DNA or high molecular weight genomic DNA.5 The CaCl2-mediated electroporation of E. coli with the plasmid DNA pBR322 has been studied.6 A protocol for the concentration of virus vectors that uses CaCl2 has been published.7CaCl2 has been used in the stabilization and two-dimensional crystallization of the NADH-ubiquinone oxidoreductase (complex I) from Escherichia coli.8 The crystallization of porcine pancreatic elastase in the presence of CaCl2 in the presence of sodium citrate reveals binding of calcium in the metal binding site of the protein.9 The effect of CaCl2 on the total fluorescence in the polymerication of the tubulin-like FtsZ division protein of Escherichia coli has been studied by a multiwell fluorescent assay.10

Application

This product is insect cell culture tested (0.5 mg/ml) and is appropriate for insect cell culture applications. Calcium chloride is used in the preparation and transformation of competent E. coli and in the
transfection of eukaryotic cells with either plasmid DNA or high molecular weight genomic DNA. The CaCl2-mediated electroporation of E. coli with the plasmid DNA pBR322 has been studied. A protocol for the concentration of virus vectors that uses CaCl2 has been published.

Preparation Note

This product is soluble in water (222 mg/ml, 2 M), yielding a clear, colorless solution.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319

Hazard Classifications

Eye Irrit. 2

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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J Philippe et al.
International journal of obesity (2005), 39(2), 295-302 (2014-06-04)
A significant proportion of severe familial forms of obesity remain genetically elusive. Taking advantage of our unique cohort of multigenerational obese families, we aimed to assess the contribution of rare mutations in 29 common obesity-associated genes to familial obesity, and
Michael A Goren et al.
Nature communications, 5, 5115-5115 (2014-10-09)
Opsin, the rhodopsin apoprotein, was recently shown to be an ATP-independent flippase (or scramblase) that equilibrates phospholipids across photoreceptor disc membranes in mammalian retina, a process required for disc homoeostasis. Here we show that scrambling is a constitutive activity of
Maheswari Jayakannan et al.
Journal of experimental botany, 66(7), 1865-1875 (2015-01-24)
The role of endogenous salicylic acid (SA) signalling cascades in plant responses to salt and oxidative stresses is unclear. Arabidopsis SA signalling mutants, namely npr1-5 (non-expresser of pathogenesis related gene1), which lacks NPR1-dependent SA signalling, and nudt7 (nudix hydrolase7), which
Mohsen Akbari et al.
Advanced functional materials, 24(26), 4060-4067 (2014-11-21)
The fabrication of cell-laden structures with anisotropic mechanical properties while having a precise control over the distribution of different cell types within the constructs is important for many tissue engineering applications. Automated textile technologies for making fabrics allow simultaneous control
Naoya Nishitani et al.
The international journal of neuropsychopharmacology, 17(8), 1321-1326 (2014-05-24)
Several lines of evidence indicate that ketamine has a rapid antidepressant-like effect in rodents and humans, but underlying mechanisms are unclear. In the present study, we investigated the effect of ketamine on serotonin (5-HT) release in the rat prefrontal cortex
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