MABT523
Anti-beta-Actin Antibody, clone RM112
clone RM112, from rabbit
Synonym(s):
Actin, cytoplasmic 1, Beta-actin, beta-Actin
Sign Into View Organizational & Contract Pricing
All Photos(2)
About This Item
Recommended Products
biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
RM112, monoclonal
species reactivity
human
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ACTB(60)
General description
Actin is a 43 kDa highly conserved structural protein. There are three main actin isotypes (alpha, beta and gamma) which show >90% amino-acid (aa) homology between isotypes and >98% homology within members of a particular isotypic group. The majority of the isotype heterogeneity is located in the amino-terminal 30 aa. The different actin isotypes have been shown to behave very differently in vitro and in vivo. Recent studies describe differential subcellular localization of gamma-actin and isotype specific binding of actin associated proteins. Beta actin is one of the two nonmuscle cytoskeletal actins and is relatively stable and appears to remain at normal levels regardless of experimental treatment. It is generally used as an internal control for experiments.
Immunogen
A peptide corresponding to the N-terminus of beta-Actin.
Application
Immunocytochemistry Analysis (ICC): A 1:200 dilution from a representative lot detected beta-Actin in A431 cells.
This Anti-beta-Actin Antibody, clone RM112 is validated for use in Western Blotting, Immunocytochemistry for the detection of beta-Actin.
Quality
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected beta-Actin in A431 cell lysate.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected beta-Actin in A431 cell lysate.
Target description
~45 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Other Notes
Concentration: Please refer to lot specific datasheet.
Not finding the right product?
Try our Product Selector Tool.
recommended
Product No.
Description
Pricing
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
PloS one, 11(5), e0155190-e0155190 (2016-05-14)
Perfluorinated chemicals (PFCs) are ubiquitously distributed in the environments including stainless pan-coating, raincoat, fire extinguisher, and semiconductor products. The PPAR family has been shown to contribute to the toxic effects of PFCs in thymus, immune and excretory systems. Herein, we
Cell transplantation, 30, 9636897211054481-9636897211054481 (2021-11-11)
Biological and cellular interleukin-6 (IL-6)-related therapies have been used to treat severe COVID-19 pneumonia with hyperinflammatory syndrome and acute respiratory failure, which prompted further exploration of the role of IL-6 in human umbilical cord mesenchymal stem cell (hUCMSC) therapy. Peripheral
Kruppel-family zinc finger proteins as emerging epigenetic biomarkers in head and neck squamous cell carcinoma.
Journal of Otolaryngology - Head & Neck Surgery = Le Journal D'Oto-rhino-laryngologie et de Chirurgie Cervico-Faciale, 52, 41-41 (2023)
Sleep, 46(9) (2023-05-08)
Long-term use of sodium oxybate (SXB), (also called gamma-hydroxybutyrate [GHB]) attenuates the cataplexy and sleepiness of human narcolepsy. We had previously found that chronic opiate usage in humans and long-term opiate administration to mice significantly increased the number of detected
Oncology letters, 17(1), 638-645 (2019-01-19)
Trichostatin A (TSA), a hydroxamate histone deacetylase inhibitor, is a compound that has been identified to induce anticancer activity. The aim of the present study was to investigate whether sorafenib, in combination with TSA, was able to augment the anticancer
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service