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  • Translationally-controlled tumor protein activates the transcription of Oct-4 in kidney-derived stem cells.

Translationally-controlled tumor protein activates the transcription of Oct-4 in kidney-derived stem cells.

Experimental and therapeutic medicine (2017-01-27)
Ying Jing, Liang-Liang He, Chang-Lin Mei
ABSTRACT

The molecular mechanisms underlying translationally-controlled tumor protein (TCTP) in the activation of octamer-binding transcription factor 4 (Oct-4) in kidney-derived stem cells have not been characterized. The aim of the present study was to identify the transcriptional activation of Oct-4 by TCTP in kidney-derived stem cells. Homology-directed repair cDNA inserted into Fisher 344 transgenic (Tg) rats and the mouse strain 129/Svj were used for the experiments. Diphtheria toxin (DT; 10 ng/kg) injected into the Tg rats created the kidney injury, which was rapidly restored by the activation of kidney-derived stem cells. Kidney-derived stem cells were isolated from the DT-injured Tg rats using cell culture techniques. The co-expression of Oct-4 and TCTP were observed in the isolated kidney-derived stem cells. Immunoblotting and reverse transcription-polymerase chain reaction analysis of TCTP null mutant (TCTP

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-OCT4/POU5F1 antibody produced in goat, affinity isolated antibody, buffered aqueous solution
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Monoclonal Anti-β-Actin antibody produced in mouse, clone AC-74, ascites fluid
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Trypsin inhibitor from chicken egg white, Type II-O, Partially purified ovomucoid, containing ovoinhibitor
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Diphtheria Toxin from Corynebacterium diphtheriae, lyophilized powder, Product is in unnicked form