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Merck

Specific indication of hemoproteins in polyacrylamide gels using a double-staining process.

Analytical biochemistry (1984-02-01)
R T Francis, R R Becker
RÉSUMÉ

Hemoproteins were revealed in polyacrylamide gels in the presence of sodium dodecyl sulfate by staining with different benzidine derivatives. When the protein samples were treated with either beta-mercaptoethanol or dithiothreitol, a significant decrease in peroxidase activity of the proteins possessing noncovalently bound heme led to diminished staining. However, when Coomassie blue R-250 staining followed the hemespecific stain it was observed that the hemoprotein bands stained more intensely than duplicate sample bands that had been stained only with the Coomassie blue R-250. This staining property allows the indication of hemoproteins in gels even after the peroxidase yield has been significantly depleted by reducing agents.

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Supelco
3,3′-Diaminobenzidine tetrahydrochloride hydrate, for spectrophotometric det. of Se, ≥97.5%