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Reduction of photo bleaching and long term archiving of chemically cleared GFP-expressing mouse brains.

PloS one (2014-12-03)
Klaus Becker, Christian Markus Hahn, Saiedeh Saghafi, Nina Jährling, Martina Wanis, Hans-Ulrich Dodt
RÉSUMÉ

Tissue clearing allows microscopy of large specimens as whole mouse brains or embryos. However, lipophilic tissue clearing agents as dibenzyl ether limit storage time of GFP-expressing samples to several days and do not prevent them from photobleaching during microscopy. To preserve GFP fluorescence, we developed a transparent solid resin formulation, which maintains the specimens' transparency and provides a constant signal to noise ratio even after hours of continuous laser irradiation. If required, high-power illumination or long exposure times can be applied with virtually no loss in signal quality and samples can be archived for years.

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Sigma-Aldrich
5-Amino-1,3,3-trimethylcyclohexanemethylamine, mixture of cis and trans, ≥99%