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Comprehensive in vivo identification of the c-Myc mRNA protein interactome using HyPR-MS.

RNA (New York, N.Y.) (2019-07-13)
Michele Spiniello, Maisie I Steinbrink, Anthony J Cesnik, Rachel M Miller, Mark Scalf, Michael R Shortreed, Lloyd M Smith
RÉSUMÉ

Proteins bind mRNA through their entire life cycle from transcription to degradation. We analyzed c-Myc mRNA protein interactors in vivo using the HyPR-MS method to capture the crosslinked mRNA by hybridization and then analyzed the bound proteins using mass spectrometry proteomics. Using HyPR-MS, 229 c-Myc mRNA-binding proteins were identified, confirming previously proposed interactors, suggesting new interactors, and providing information related to the roles and pathways known to involve c-Myc. We performed structural and functional analysis of these proteins and validated our findings with a combination of RIP-qPCR experiments, in vitro results released in past studies, publicly available RIP- and eCLIP-seq data, and results from software tools for predicting RNA-protein interactions.

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Sigma-Aldrich
IgG de lapin normale, Normal Rabbit IgG Polyclonal Antibody control validated for use in Immunoprecipitation & Western Blotting.
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Sigma-Aldrich
Kit d'immunoprécipitation des protéines de liaison à l'ARN (forme réticulée) EZ-Magna NuCLEAR RIP, EZ-Magna Nuclear RIP (Cross-Linked) RNA-Binding Protein Immunoprecipitation Kit is designed for the analysis of chromatin associated RNA such lncRNAs, enhancer RNAs and miRNAs.
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