S5942
SYPRO® Tangerine Protein Gel Stain
About This Item
Produits recommandés
Durée de conservation
≥6 mo. (when stored at room temperature and protected from light.)
Fluorescence
λex 300,490 nm; λem 640 nm
Description générale
SYPRO® Tangerine Protein Gel Stain is:
- Highly sensitive. The stain can detect 4 to 8 ng of protein per minigel band, making it more sensitive than Coomassie® Brilliant Blue staining and as sensitive as many silver staining techniques.
- Rapid. Staining is complete in less than one hour.
- Simple. After electrophoresis, the gel is stained, rinsed and photographed; no fixation or destaining steps are required.
- Compatible with standard laboratory equipment. Stained proteins can be visualized with a standard 300 nm UV transilluminator or laser scanner.
- Cost-effective. Staining with SYPRO Tangerine is less expensive than silver staining and requires less time.
- Low protein-to protein variability. The dye interacts with the SDS coat around the protein, giving more consistent staining between different types of proteins compared to Coomassie or silver staining.
- Selective for proteins. SYPRO Tangerine detects proteins as small as 6.5 kDa and does not stain nucleic acids or lipopolysaccharides.
- Broad linear range of detection. The stain interacts with the SDS-protein micelle during gel staining at a fairly constant stoichiometry of 1.4 to 1, such that the fluorescence intensity of the stained bands is linear with protein quantity over three orders of magnitude (a much broader range than either Coomassie or silver staining).
Attention
Informations légales
Produit(s) apparenté(s)
Classe de danger pour l'eau (WGK)
WGK 3
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