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Key Documents

11796895001

Roche

DIG Easy Hyb Granules

greener alternative

powder, suitable for hybridization

Synonyme(s) :

DIG system

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About This Item

Code UNSPSC :
41105326

Agence

suitable for

Niveau de qualité

Forme

powder

Utilisation

sufficient for 6 x 100 mL

Fabricant/nom de marque

Roche

Conditions de stockage

(Keep container tightly closed in a dry and well-ventilated place.)

Caractéristiques du produit alternatif plus écologique

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

Technique(s)

hybridization: suitable

Couleur

white

pH

7,0

Solubilité

water: soluble

Adéquation

suitable for nucleic acid hybridization

Autre catégorie plus écologique

Température de stockage

20-25°C

Description générale

DIG Easy Hyb is a hybridization solution used for nucleic acid blots with digoxigenin-labeled probes. DIG Easy Hyb does not contain formamide but facilitates the lowering of hybridization temperature comparable to buffers that contain 50% formamide.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Application

DIG Easy Hyb Granules can be used in all membrane hybridization applications using nonradioactive (e.g., digoxigenin-labeled) nucleic acid probes. It is specifically designed for use with DIG kits.

Caractéristiques et avantages

  • DIG Easy Hyb is a convenient ready-to-use hybridization buffer, saving time and effort.
  • DIG Easy Hyb aligns with the principles of Green Chemistry by offering a greener alternative for hybridization buffers.

Notes préparatoires

Working solution: Add carefully 64 ml sterile double-dist. water to the plastic bottle, dissolve by stirring immediately for 5 minutes at 37 °C. The availability of 6 x 100 ml portions allows you to freshly "produce" the DIG Easy Hyb when required—no long-term storage of a solution is required.
Note: For applications with Northern blots, DIG Easy Hyb Granules must be dissolved under RNase-free conditions.
Nontoxic granules, aliquoted in plastic bottles. Reconstitute to provide 100ml of a 1x solution, ready-to-use.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Informations légales

DIG Easy Hyb is a trademark of Roche

Pictogrammes

Exclamation mark

Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Eye Irrit. 2 - Skin Irrit. 2

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

does not flash

Point d'éclair (°C)

does not flash


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

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An Li et al.
Fungal genetics and biology : FG & B, 48(6), 602-611 (2011-02-18)
Several Aspergillus species are well-known for the production of a variety of organic acids. In this study, a cloned based transcriptomics approach was used to identify genes crucial in the biosynthesis pathway for one of these acids, itaconic acid. From
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RNA biology, 11(11), 1467-1478 (2014-12-09)
6S RNA is a highly abundant small non-coding RNA widely spread among diverse bacterial groups. By competing with DNA promoters for binding to RNA polymerase (RNAP), the RNA regulates transcription on a global scale. RNAP produces small product RNAs derived
Yanchang Luo et al.
Plant biotechnology (Tokyo, Japan), 37(1), 69-75 (2020-05-05)
Retrotransposons are mobile genetic elements capable of transposition via reverse transcription of RNA intermediates. Rice cultivar Nipponbare contains two nearly identical genomic copies of Tos17, an endogenous copia-like LTR retrotransposon, on chromosomes 7 (Tos17 Chr.7) and 10 (Tos17 Chr.10), respectively.
Koichi Kitao et al.
Retrovirology, 18(1), 36-36 (2021-11-11)
Retroviruses utilize multiple unique RNA elements to control RNA processing and translation. However, it is unclear what functional RNA elements are present in endogenous retroviruses (ERVs). Gene co-option from ERVs sometimes entails the conservation of viral cis-elements required for gene

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