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Merck
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Key Documents

MABF152C

Sigma-Aldrich

Anti-F4/80 (mouse), clone BM8.1, biotin conjugate Antibody

clone BM8.1, from rat, purified by affinity chromatography

Synonyme(s) :

EGF-like module-containing mucin-like hormone receptor-like 1, Cell surface glycoprotein F4/80, EGF-like module receptor 1, EMR1 hormone receptor, F4/80

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.45

Source biologique

rat

Niveau de qualité

100

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

BM8.1, monoclonal

Produit purifié par

affinity chromatography

Espèces réactives

mouse

Conditionnement

antibody small pack of 25 μg

Technique(s)

flow cytometry: suitable

Numéro d'accès UniProt

Q61549

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

mouse ... Adgre1(13733)

Description générale

The BM8.1 antibody is specific for mouse F4/80 antigen, a 125 kDa transmembrane protein widely expressed by members of the mononuclear phagocyte system and considered to be a key marker for mature macrophage cells. F4/80 is differentially expressed during myeloid cell development, and may be regulated by certain cytokines within the tissue microenvironment. Other cell types shown to express this antigen include Langerhans cells, Kupffer cells and dendritic cell subsets. BM8.1 is widely used together with antibodies to CD115 (c-fms), CD11b and CD11c to identify myeloid / macrophage cells by flow cytometry.

Immunogène

Immunogen information for this antibody is proprietary information.

Application

Anti-F4/80 (mouse), clone BM8.1, biotin conjugate Antibody detects level of F4/80 and has been published and validated for use in Flow Cytometry.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology

Qualité

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.125 μg from a representative lot detected F4/80 in one million C57Bl/6 bone marrow cells.

Forme physique

Purified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
Purified by size-exclusion chromatography

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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