SAB4300645

Anti-GAPDH antibody produced in rabbit

affinity isolated antibody

• Synonym(s): Anti-G3PD, Anti-GAPD, Anti-MGC88685
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: ~37 kDa
• species reactivity: human, rat, mouse
• concentration: 1 mg/mL
• technique(s): western blot: 1:500-1:1000
• isotype: IgG
• immunogen sequence: (P-A-K-Y-D)
• NCBI accession no.: NM_002046.3
• UniProt accession no.: P04406
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... GAPDH(2597)

Description

General description

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a multifunctional protein , that is located on chromosome 12p13. It is basically called as a glycolytic enzyme.

The antibody detects endogenous level of total GAPDH protein.

Immunogen

Peptide sequence around aa. 252-256 (P-A-K-Y-D), according to the protein NP_002037.2

Application

Anti-GAPDH antibody has been used in western blotting.{60,61}

Biochem/physiol Actions

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) plays an important role in DNA repair. It is essential for the sixth step of glycolysis. It participates in transcriptional and posttranscriptional gene regulation. It also controls intracellular membrane trafficking and cell death. Accumulation of GAPDH results in mitochondrial dysfunction.

Features and Benefits

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Target description

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC By similarity. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.

Physical form

Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable