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Key Documents

GE28-9031-33

Protein A High Performance MultiTrap™

Cytiva 28-9031-33

Synonym(s):

High Performance Protein A, MultiTrap Protein A

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About This Item

UNSPSC Code:
41105323
NACRES:
NA.25

ligand

native protein A

material

polypropylene and polyethylene

feature

wetted part: no

packaging

pkg of 4 ea

manufacturer/tradename

Cytiva 28-9031-33

storage condition

(20% Ethanol)

parameter

30 °C max. temp.
4 °C min. temp.

size

127.8 mm × 85.5 mm × 30.6 mm

matrix

highly cross-linked 6% agarose

avg. part. size

34 μm

cleaning

2-10

working range

3-9

storage temp.

2-8°C

Related Categories

General description

Protein A Sepharose™ High Performance has high affinity for the Fc region of IgG from a variety of species.

Protein A Sepharose™ High Performance has high affinity for the Fc region of IgG from a variety of species. The two options in the protein enrichment protocol allow the attached antigen (protein of interest) to be eluted separately (cross-link protocol) or together with the antibody (classic protocol). Remember to order the collection plates for washing and elution of the enriched protein.

There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Features and Benefits

  • Fast, reliable ease-of-use format for small-scale purification of antibodies or enrichment of proteins of interest in prepacked 96-well filter plates that are ready to use.
  • Flexible format, with protocols for classic elution and cross-link method.
  • Elution conditions formatted for both electrophoresis and LC-MS analysis workflows.
  • Fast binding kinetics and high capacity provides high yield.
  • Easy scale-up with HiTrap® Protein A HP prepacked columns.
  • Prepacked with Protein A Sepharose™ High Performance for coupling of antibodies of IgG subclasses.
  • MultiTrap™ 96-well filter plates can be used with centrifugation or vacuum, manually or in automated robotic systems.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HiTrap is a registered trademark of Cytiva
MultiTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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