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C5572

Sigma-Aldrich

Click′s Medium

With sodium bicarbonate, without mercaptoethanol and L-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Click’s EHAA Medium, Eagle’s Ham’s amino acids; EHAA

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

L-glutamine: no
phenol red: yes
glucose: yes

shipped in

ambient

storage temp.

2-8°C

Application

Click′s Medium has been used:
  • to incubate cartilage discs to measure the ability of chondrocytes to synthesize sulphated glycosaminoglycans (GAGs) by a metabolic assay
  • as a culture medium to incubate frozen peripheral blood mononuclear cells (PBMCs) in vitro stimulation and evaluation of immunologic response
  • for the suspension, washing and culture of T-cells

Reconstitution

Supplement with 0.584 g/L L-glutamine.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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David E Pegg et al.
Cryobiology, 93, 12-17 (2020-04-25)
Although it is relatively straightforward to cryopreserve living isolated chondrocytes, at the present time there is no satisfactory method to preserve surgical grafts between the time of procurement or manufacture and actual use. In earlier papers we have established that
David E Pegg et al.
Cryobiology, 52(3), 347-359 (2006-03-11)
Although isolated chondrocytes can be cryopreserved by standard methods, at the present time there is no satisfactory method that will preserve living chondrocytes in situ in surgical grafts, between the time of procurement or manufacture and actual use; survival of
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