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Carbonic Anhydrase I from human erythrocytes

Name: Carbonic Anhydrase I from human erythrocytes
Brand: SIGMA

Synonym(s):

Carbonate Dehydratase, Carbonate Hydrolyase, Carbonic Anhydrase Isozyme I

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About This Item

CAS Number:

9001-03-0

UNSPSC Code:

12352204

NACRES:

NA.25

EC Number:

232-576-6

MDL number:

MFCD00081467

EC Number:

4.2.1.1 (BRENDA, IUBMB)

Specific activity:

100-500 W-A units/mg protein

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Properties

form

powder

Quality Segment

200

specific activity

100-500 W-A units/mg protein

mol wt

30 kDa

color

white

pI

~6.6

UniProt accession no.

P00915

storage temp.

−20°C

Gene Information

human ... CA1(759)

Description

Application

Carbonic anhydrase from human erythrocytes (HCA) has been used to study the molten-globule state of carbonic anhydrase (CA). Chaperone-like α-crystallin binds to this state of the enzyme and prevents its aggregation.[1] The enzyme from sigma has been used for the analysis of thermodynamic stability of the enzyme.[2] Furthermore, its clinical significance has been evaluated in human non-small cell lung cancer.[3]

Biochem/physiol Actions

Carbonic anhydrase is a zinc metalloenzyme that has a molecular weight of approximately 30,000 Da. The enzyme catalyzes the hydration of carbon dioxide to carbonic acid. It is involved in vital processes such as pH and CO₂ homeostasis, transport of bicarbonate and CO₂, biosynthetic reactions, bone resorption, calcification, and tumorigenicity. Therefore, this enzyme is an important target for inhibitors with clinical applications in various pathologies such as glaucoma, epilepsy and Parkinson′s disease.[2]

Preparation Note

chromatographically
purified.

Other Notes

One Wilbur-Anderson (W-A) unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0 °C. (One W-A unit is essentially equivalent to one Roughton-Booth unit.)

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This Item	C5024	C3934	C2624
Sigma-Aldrich C4396 Carbonic Anhydrase I from human erythrocytes	Sigma-Aldrich C5024 Carbonic Anhydrase from bovine erythrocytes	Sigma-Aldrich C3934 Carbonic Anhydrase from bovine erythrocytes	Sigma-Aldrich C2624 Carbonic Anhydrase from bovine erythrocytes
Gene Information human ... CA1(759)	Gene Information -	Gene Information -	Gene Information -
specific activity 100-500 W-A units/mg protein	specific activity -	specific activity ≥2,000 W-A units/mg protein	specific activity ≥3,500 W-A units/mg protein
form powder	form lyophilized powder	form lyophilized powder	form lyophilized powder
UniProt accession no. P00915	UniProt accession no. -	UniProt accession no. -	UniProt accession no. -
mol wt 30 kDa	mol wt ~29 kDa	mol wt 30 kDa	mol wt -
storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. 2-8°C