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SLM-202

Sigma-Aldrich

Dulbecco′s Modified Eagle′s Media 2X, With 4,500 mg/L Glucose and L-Glutamine, without NaHCO3 or Sodium Pyruvate

Synonym(s):

DMEM 2X

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About This Item

UNSPSC Code:
12352207
eCl@ss:
32160801
NACRES:
NA.75

description

The Dulbecco′s Modified Eagle′s Media 2X, With 4,500 mg/L Glucose & L-Glutamine, without NaHCO3 or Sodium Pyruvate is available in a 500 mL format & has been optimized & validated for cell culture.

Quality Level

200

form

liquid

manufacturer/tradename

Specialty Media

technique(s)

cell culture | mammalian: suitable

input

sample type: mouse embryonic stem cell(s)
sample type epithelial cells
sample type induced pluripotent stem cell(s)
sample type neural stem cell(s)
sample type hematopoietic stem cell(s)
sample type pancreatic stem cell(s)
sample type: human embryonic stem cell(s)
sample type cardiac stem cell(s)
sample type mesenchymal stem cell(s)

General description

The Dulbecco′s Modified Eagle′s Media 2X, With 4,500 mg/L Glucose & L-Glutamine, without NaHCO3 or Sodium Pyruvate is available in a 500 mL format. It is optimized and validated for use in cell culture.

Application

Dulbecco′s Modified Eagle′s Media 2X, With 4,500 mg/L Glucose and L-Glutamine, without NaHCO3 or Sodium Pyruvate has been used:
  • in a well plate to mix transformed or parental infrared fluorescent protein (iRFP) 3T3 fibroblasts for soft agar assay
  • to prepare collagen solution for collagen gel contraction
  • in the preparation of 5% methyl cellulose

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Robert S McNeill et al.
Journal of visualized experiments : JoVE, (90), e51763-e51763 (2014-08-26)
Current astrocytoma models are limited in their ability to define the roles of oncogenic mutations in specific brain cell types during disease pathogenesis and their utility for preclinical drug development. In order to design a better model system for these
Pugazendhi Erusappan et al.
Scientific reports, 9(1), 15283-15283 (2019-10-28)
Integrin α11β1 is a collagen-binding integrin, which is receiving increasing attention in the context of wound healing and fibrosis. Although α11β1 integrin displays similar collagen specificity to α2β1 integrin, both integrins have distinct in vivo functions. In this context, the
Viola Paulus-Hock et al.
PloS one, 9(6), e98399-e98399 (2014-06-03)
Anchorage independent growth is one of the hallmarks of oncogenic transformation. Here we show that infrared fluorescent protein (iRFP) based assays allow accurate and unbiased determination of colony formation and anchorage independent growth over time. This protocol is particularly compatible

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