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Merck
  • Host-pathogen interactions in bovine mammary epithelial cells and HeLa cells by Staphylococcus aureus isolated from subclinical bovine mastitis.

Host-pathogen interactions in bovine mammary epithelial cells and HeLa cells by Staphylococcus aureus isolated from subclinical bovine mastitis.

Journal of dairy science (2017-06-03)
Ivana G Castilho, Stéfani Thais Alves Dantas, Hélio Langoni, João P Araújo, Ary Fernandes, Fernanda C L Alvarenga, Leandro Maia, Didier Q Cagnini, Vera L M Rall
ZUSAMMENFASSUNG

Staphylococcus aureus is a common pathogen that causes subclinical bovine mastitis due to several virulence factors. In this study, we analyzed S. aureus isolates collected from the milk of cows with subclinical mastitis that had 8 possible combinations of bap, icaA, and icaD genes, to determine their capacity to produce biofilm on biotic (bovine primary mammary epithelial cells and HeLa cells) and abiotic (polystyrene microplates) surfaces, and their ability to adhere to and invade these cells. We also characterized isolates for microbial surface components recognizing adhesive matrix molecules (MSCRAMM) and agr genes, and for their susceptibility to cefquinome sulfate in the presence of biofilm. All isolates adhered to and invaded both cell types, but invasion indexes were higher in bovine primary mammary epithelial cells. Using tryptic soy broth + 1% glucose on abiotic surfaces, 5 out of 8 isolates were biofilm producers, but only the bap

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Sigma-Aldrich
Triton X-100, laboratory grade
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BB-0496-1G1 g
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585,00 $
BB-0496-5G5 g
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Supelco
Cefquinom-sulfat, VETRANAL®, analytical standard
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
SKULieferanten-SKUPackungsgrößeVerfügbarkeitPreisMenge
BB-0496-1G1 g
Versand in 7 Tagen - ab Aldrich Partner.
585,00 $
BB-0496-5G5 g
Versand in 7 Tagen - ab Aldrich Partner.
2.340,00 $