Direkt zum Inhalt
Merck
  • Antibacterial properties of 2% lidocaine and reduced rate of endophthalmitis after intravitreal injection.

Antibacterial properties of 2% lidocaine and reduced rate of endophthalmitis after intravitreal injection.

Retina (Philadelphia, Pa.) (2013-10-10)
Aaron Tustin, Stephen J Kim, Amy Chomsky, G Baker Hubbard, Jinsong Sheng
ZUSAMMENFASSUNG

To determine whether the application of subconjunctival 2% lidocaine/0.1% methylparaben for anesthesia may reduce rates of endophthalmitis after intravitreal (IVT) injection. We performed in vitro experiments to determine the antibacterial properties of 2% lidocaine/0.1% methylparaben (lidocaine) against causative organisms of endophthalmitis. Isolates of Staphylococcus aureus, Staphylococcus epidermidis, and Streptococcus viridans from patients with endophthalmitis were incubated with or without lidocaine. Aliquots (100 µL) were plated on Mueller-Hinton (S. aureus and S. epidermidis) or blood agar plates (S. viridans) at 0, 10, 30, 120, and 240 minutes, and colonies were counted after 24 hours. A retrospective review of 15,042 IVT injections was performed from January 2004 to February 2011 to determine the rate of endophthalmitis with or without application of subconjunctival lidocaine for anesthesia. Lidocaine demonstrated rapid bactericidal effects against all 3 organisms. After 10 minutes of exposure, there was approximately a 90% (P < 0.01), 95% (P < 0.001), and 92% (P < 0.001) reduction in colony forming units when compared with time 0 for S. aureus, S. epidermidis, and S. viridans, respectively. Complete elimination of colony forming units occurred at subsequent time points for each organism in contrast to logarithmic increase for control plates. There were a total of 0 cases of endophthalmitis of 6,853 IVT injections performed with subconjunctival lidocaine and 8 cases of endophthalmitis of 8,189 (0.1%) IVT injections performed with other methods of anesthesia (P = 0.03). Application of subconjunctival 2% lidocaine/0.1% methylparaben for anesthesia may reduce the incidence of endophthalmitis after IVT injection.

MATERIALIEN
Produktnummer
Marke
Produktbeschreibung

Sigma-Aldrich
Lidocain, powder
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
USP
Methylparaben, United States Pharmacopeia (USP) Reference Standard
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Supelco
Methylparaben, PESTANAL®, analytical standard
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
USP
Lidocain, United States Pharmacopeia (USP) Reference Standard
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Sigma-Aldrich
Methyl-4-hydroxybenzoat, BioReagent, suitable for insect cell culture
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Supelco
Methylparaben, Pharmaceutical Secondary Standard; Certified Reference Material
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Sigma-Aldrich
Methyl-4-hydroxybenzoat, ≥99%, FCC
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Sigma-Aldrich
Lidocain, analytical standard
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Sigma-Aldrich
Methyl-4-hydroxybenzoat, ReagentPlus®, ≥99.0%, crystalline
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Supelco
Lidocain, Pharmaceutical Secondary Standard; Certified Reference Material
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Methylparahydroxybenzoat, European Pharmacopoeia (EP) Reference Standard
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Sigma-Aldrich
Methyl-4-hydroxybenzoat, BioXtra, ≥99.0% (titration)
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Sigma-Aldrich
Methyl-4-hydroxybenzoat, analytical standard
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Sigma-Aldrich
Methyl-4-hydroxybenzoat, tested according to Ph. Eur.
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Lidocain, European Pharmacopoeia (EP) Reference Standard
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge