Direkt zum Inhalt
Merck
  • Defining the localization and molecular characteristic of minor salivary gland label-retaining cells.

Defining the localization and molecular characteristic of minor salivary gland label-retaining cells.

Stem cells (Dayton, Ohio) (2014-04-10)
Hongjun Zhang, Keerthi Boddupally, Eve Kandyba, Krzysztof Kobielak, Yibu Chen, Sutao Zu, Rashi Krishnan, Uttam Sinha, Agnieszka Kobielak
ZUSAMMENFASSUNG

Adult stem cells (SCs) are important to maintain homeostasis of tissues including several mini-organs like hair follicles and sweat glands. However, the existence of stem cells in minor salivary glands (SGs) is largely unexplored. In vivo histone2B green fluorescent protein pulse chase strategy has allowed us to identify slow-cycling, label-retaining cells (LRCs) of minor SGs that preferentially localize in the basal layer of the lower excretory duct with a few in the acini. Engraftment of isolated SG LRC in vivo demonstrated their potential to differentiate into keratin 5 (basal layer marker) and keratin 8 (luminal layer marker)-positive structures. Transcriptional analysis revealed activation of TGFβ1 target genes in SG LRC and BMP signaling in SG progenitors. We also provide evidence that minor SGSCs are sensitive to tobacco-derived tumor-inducing agent and give rise to tumors resembling low grade adenoma. Our data highlight for the first time the existence of minor SG LRCs with stem cells characteristic and emphasize the role of transforming growth factor beta (TGFβ) pathway in their maintenance.

MATERIALIEN
Produktnummer
Marke
Produktbeschreibung

Sigma-Aldrich
Tamoxifen, ≥99%
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Sigma-Aldrich
Propylenglycol, ≥99.5% (GC), FCC, FG
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
USP
Propylenglycol, United States Pharmacopeia (USP) Reference Standard
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Sigma-Aldrich
1,2-Propandiol, ACS reagent, ≥99.5%
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Sigma-Aldrich
Propylenglykol, meets USP testing specifications
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Supelco
Propylenglykol, Pharmaceutical Secondary Standard; Certified Reference Material
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Sigma-Aldrich
1,2-Propandiol, ReagentPlus®, 99%
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Sigma-Aldrich
1,2-Propandiol, puriss. p.a., ACS reagent, ≥99.5% (GC)
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Supelco
Tamoxifen, analytical standard
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Supelco
1,2-Propandiol, analytical standard
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Sigma-Aldrich
1,2-Propandiol, meets analytical specification of Ph. Eur., BP, USP, ≥99.5%
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Propylenglycol, European Pharmacopoeia (EP) Reference Standard
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Sigma-Aldrich
1,2-Propandiol, tested according to Ph. Eur.
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge