Direkt zum Inhalt
Merck
  • Hesperetin induces the apoptosis of hepatocellular carcinoma cells via mitochondrial pathway mediated by the increased intracellular reactive oxygen species, ATP and calcium.

Hesperetin induces the apoptosis of hepatocellular carcinoma cells via mitochondrial pathway mediated by the increased intracellular reactive oxygen species, ATP and calcium.

Medical oncology (Northwood, London, England) (2015-03-05)
Jixiang Zhang, Jia Song, Dandan Wu, Jing Wang, Weiguo Dong
ZUSAMMENFASSUNG

Hesperetin, a flavonoid from citrus fruits, has been proved to possess biological activity on various types of human cancers. However, few related studies on hepatocellular carcinoma are available. In this study, we aimed to investigate the effect of hesperetin on hepatocellular carcinoma cells in vitro and in vivo and clarify its potentially specific mechanism. Compared with the control group, the proliferations of hepatocellular carcinoma cells in hesperetin groups were significantly inhibited (P < 0.05), and a dose- and time-dependent inhibition of cell viability was observed. When pretreated with H2O2 (1 mM) or N-acetyl-L-cysteine (5 mM), the inhibition of cell viability by hesperetin was enhanced or reduced, respectively (P < 0.05). Similarly, the levels of intracellular ROS, ATP and Ca(2+) changed in different groups (P < 0.05). The results of Hoechst 33258 staining showed that the percentages of apoptotic cells in hesperetin groups are remarkably higher than that in control group (P < 0.05). And the results of Western blot showed that hesperetin caused an increase in the levels of cytosolic AIF, cytosolic Apaf-1, cytosolic Cyt C, caspase-3, caspase-9 and Bax and a decrease in that of Bcl-2, mitochondrial AIF, mitochondrial Apaf-1 and mitochondrial Cyt C (P < 0.05). Meanwhile, hesperetin significantly inhibited the growth of xenograft tumors. Our study suggests that hesperetin could inhibit the proliferation and induce the apoptosis of hepatocellular carcinoma via triggering the activation of the mitochondrial pathway by increasing the levels of intracellular ROS, ATP and Ca(2+).

MATERIALIEN
Produktnummer
Marke
Produktbeschreibung

Sigma-Aldrich
Wasserstoffperoxid -Lösung, contains inhibitor, 30 wt. % in H2O, ACS reagent
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Sigma-Aldrich
Wasserstoffperoxid -Lösung, 30 % (w/w) in H2O, contains stabilizer
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Sigma-Aldrich
Wasserstoffperoxid -Lösung, 50 wt. % in H2O, stabilized
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Sigma-Aldrich
Phenylmethansulfonylfluorid, ≥98.5% (GC)
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Sigma-Aldrich
Wasserstoffperoxid -Lösung, contains inhibitor, 35 wt. % in H2O
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Sigma-Aldrich
Phenylmethansulfonylfluorid, ≥99.0% (T)
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Millipore
Wasserstoffperoxid -Lösung, 3%, suitable for microbiology
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Sigma-Aldrich
Hesperetin, ≥95%
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Sigma-Aldrich
(Tyr[SO3H]27)Cholecystokinin-Fragment 26-33 Amid, ≥97% (HPLC), powder
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Sigma-Aldrich
Wasserstoffperoxid -Lösung, 34.5-36.5%
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Sigma-Aldrich
Wasserstoffperoxid -Lösung, contains inhibitor, 30 wt. % in H2O, meets USP testing specifications
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Supelco
Wasserstoffperoxid -Lösung, ≥30%, for trace analysis
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Supelco
Wasserstoffperoxid -Lösung, 30 % (w/w), for ultratrace analysis
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Sigma-Aldrich
Wasserstoffperoxid -Lösung, tested according to Ph. Eur.
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Supelco
Hesperetin, analytical standard
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge