Direkt zum Inhalt
Merck
  • Isolation, chemical characterization, and subcellular distribution of 1-O-alkyl-2-acetyl-sn-glycerol in Tetrahymena pyriformis cells.

Isolation, chemical characterization, and subcellular distribution of 1-O-alkyl-2-acetyl-sn-glycerol in Tetrahymena pyriformis cells.

Journal of biochemistry (1996-04-01)
C Tellis, D C Tsoukatos, M E Lekka
ZUSAMMENFASSUNG

1-O-Hexadecyl-2-acetyl-sn-glycerol, the immediate precursor of platelet- activating factor (PAF) in its de novo formation, was detected in the protozoon Tetrahymena pyriformis. It was purified from the total lipid extract by TLC, after successive developments in two different solvent systems. Characterization was assessed by (a) gas-liquid chromatography with electron capture detection, and (b) gas chromatography combined with mass spectrometry in selected ion monitoring mode, after derivatization with heptafluorobutyric acid anhydride and tert-butyldimethylchlorosilane/imidazole, respectively. Its quantity was found to be 0.1 nmol/10(7) cells from the GC-MS, using authentic alkylacetylglycerol as external standard. Cell fractionation revealed that alkylacetylglycerol is located exclusively in the microsomal fraction of the protozoon. Previously, we have reported the occurrence of PAF in the microsomal fraction, as well as a dithiothreitol-insensitive CDP-choline: cholinephosphotransferase activity that utilizes exogenous alkylacetylglycerol as substrate in the mitochondrial and microsomal fractions. The above findings indicate that PAF can be formed in the cell by the de novo pathway.

MATERIALIEN
Produktnummer
Marke
Produktbeschreibung

Sigma-Aldrich
tert-Butyl(chlor)dimethylsilan, reagent grade, 97%
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Sigma-Aldrich
Heptafluorbuttersäureanhydrid, ≥98%
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Supelco
Heptafluorbuttersäureanhydrid, for GC derivatization, LiChropur, ≥99.0%
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Sigma-Aldrich
tert-Butyldimethylsilylchlorid -Lösung, 1.0 M in THF
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Sigma-Aldrich
tert-Butylchlordimethylsilan -Lösung, 50 wt. % in toluene
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Supelco
Heptafluorbuttersäureanhydrid, for GC derivatization, LiChropur, ≥99.0%
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge