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  • Analysis of underivatized polyamines by reversed phase liquid chromatography with electrospray tandem mass spectrometry.

Analysis of underivatized polyamines by reversed phase liquid chromatography with electrospray tandem mass spectrometry.

Journal of pharmaceutical and biomedical analysis (2007-10-20)
Merja R Häkkinen, Tuomo A Keinänen, Jouko Vepsäläinen, Alex R Khomutov, Leena Alhonen, Juhani Jänne, Seppo Auriola
ZUSAMMENFASSUNG

A reversed phase liquid chromatography-electrospray ionization-tandem mass spectrometric method (RP-LC-ESI-MS/MS) was developed to separate and detect polyamines with minimal sample pre-treatment and without any derivatization. Prior to MS/MS analysis, a complete chromatographic separation of polyamines was achieved by a linear gradient elution using heptafluorobutyric acid as a volatile ion-pair modifier, and signal suppression was prevented by post-column addition of 75% propionic acid in isopropanol to the column flow. The developed method was successfully applied to the identification of metabolites formed from N(1),N(12)-diethylspermine in the reaction catalyzed by recombinant human polyamine oxidase and to the detection of eight different polyamines in a standard mixture.

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