Direkt zum Inhalt
Merck
  • Rapid and selective micellar electrokinetic chromatography for simultaneous determination of amikacin, kanamycin A, and tobramycin with UV detection and application in drug formulations.

Rapid and selective micellar electrokinetic chromatography for simultaneous determination of amikacin, kanamycin A, and tobramycin with UV detection and application in drug formulations.

Electrophoresis (2005-01-26)
Hsin-Hua Yeh, Shun-Jin Lin, Ju-Yun Ko, Chia-An Chou, Su-Hwei Chen
ZUSAMMENFASSUNG

A simple and selective micellar electrokinetic chromatography (MEKC) with UV detection is described for simultaneous determination of amikacin, tobramycin, and kanamycin A, performed in Tris buffer (180 mM; pH 9.1) with 300 mM sodium pentanesulfonate (SPS) as an anionic surfactant. Under this condition, good separation with high efficiency and the required short analysis time is achieved. The linear ranges of the method for the determination of amikacin, tobramycin, and kanamycin A were 0.1-0.5 mg / mL, 0.4-2.0 mg / mL, and 0.4-2.0 mg / mL, respectively; the detection limits (signal-to-noise ratio = 3; injection, 0.5 psi 5 s) were 0.08, 0.2, and 0.2 mg / mL, respectively. The small amount of sample required and the expeditiousness of the procedure allow content uniformity to be determined in individual commercial products.

MATERIALIEN
Produktnummer
Marke
Produktbeschreibung

Sigma-Aldrich
Natriumpentansulfonat, ≥95% (elemental analysis)
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Sigma-Aldrich
Natriumpentansulfonat, BioXtra
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge