Direkt zum Inhalt
Merck

Modeling retinitis pigmentosa through patient-derived retinal organoids.

STAR protocols (2021-04-27)
Yan-Ping Li, Wen-Li Deng, Zi-Bing Jin
ZUSAMMENFASSUNG

Human-induced pluripotent stem cells (hiPSCs) can be differentiated into well-structured retinal organoids. In this protocol, we successfully established 3D retinae from patient-derived hiPSCs and built the retinitis pigmentosa model in vitro. Moreover, mutation in the retinitis pigmentosa GTPase regulator (RPGR) gene was corrected by CRISPR-Cas9 gene editing, which rescued the structure and function of the 3D retinae. For complete details on the use and execution of this protocol, please refer to Deng et al. (2018).

MATERIALIEN
Produktnummer
Marke
Produktbeschreibung

Sigma-Aldrich
Dimethylsulfoxid, Hybri-Max, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Roche
DNAse I, from bovine pancreas
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Sigma-Aldrich
Retinsäure, ≥98% (HPLC), powder
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Sigma-Aldrich
MEM Nicht-essentielle Aminosäure-Lösung (100×), without L-glutamine, liquid, sterile-filtered, BioReagent, suitable for cell culture
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Sigma-Aldrich
1-Thioglycerin, liquid, BioReagent, suitable for cell culture, ≥97% (titration)
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Sigma-Aldrich
G 418, suitable for plant cell culture, BioReagent
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Sigma-Aldrich
Monoclonal Anti-Opsin antibody produced in mouse, clone RET-P1, ascites fluid
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge