Direkt zum Inhalt
Merck
  • A targeted mass spectrometry method for the accurate label-free quantification of immunogenic gluten peptides produced during simulated digestion of food matrices.

A targeted mass spectrometry method for the accurate label-free quantification of immunogenic gluten peptides produced during simulated digestion of food matrices.

MethodsX (2020-10-10)
Olivia Ogilvie, Nigel Larsen, Kevin Sutton, Laura Domigan, Juliet Gerrard, Nicholas Demarais, Sarah Roberts
ZUSAMMENFASSUNG

Mass spectrometry (MS) is an emerging method to determine the accurate concentration of immunogenic gluten peptides. It is of interest to quantify specific peptides within the gluten peptidome due to the role they play in the activation of the celiac immune cascade. Celiac disease is an autoimmune disorder triggered in genetically susceptible individuals by the presence of specific gluten peptides that resist digestion in the gastrointestinal tract. The protocol detailed within this paper can accurately quantify (label-free) the concentration of six immunogenic gluten peptides (including the 33mer) released from a food matrix using the INFOGEST in vitro digestion protocol. This method can be used to monitor small changes in the concentration of these marker peptides in response to exogenous factors such as plant-breeding, fermentation or food processing.

MATERIALIEN
Produktnummer
Marke
Produktbeschreibung

Sigma-Aldrich
Pepsin aus Schweinemagenschleimhaut, lyophilized powder, ≥2,500 units/mg protein (E1%/280)
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Sigma-Aldrich
αα-Chymotrypsin aus Rinderpankreas, Type II, lyophilized powder, ≥40 units/mg protein
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Sigma-Aldrich
Trypsin aus Schweinepankreas, Type IX-S, lyophilized powder, 13,000-20,000 BAEE units/mg protein
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge
Sigma-Aldrich
α-Amylase aus Schweinepankreas, PMSF Treated, Type I-A, saline suspension, ≥1000 units/mg protein (E1%/280)
SKU
Packungsgröße
Verfügbarkeit
Preis
Menge