L9656

Lipoprotein-Lipase aus Pseudomonas sp.

lyophilized powder, ≥50,000 units/mg solid

• Synonym(e): Diacylglycerin Lipase
• CAS-Nummer: 9004-02-8
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-669-1
• MDL number: MFCD00131523
• EG-Nummer: 3.1.1.34 (BRENDA, IUBMB)
• Specific activity: ≥50,000 units/mg solid
• Biological source: Burkholderia spp.

Eigenschaften

• biological source: Burkholderia spp.
• Quality Segment: 200
• form: lyophilized powder
• specific activity: ≥50,000 units/mg solid
• storage temp.: −20°C

Beschreibung

General description

Lipoprotein Lipase (LPL) is a glycerol ester hydrolase. Several bacteria that produce LPL, belongs to the genus Pseudomonas, Serratia and Mucor.

Lipoprotein lipase hydrolyzes triglycerides in plasma lipoproteins causing release of fatty acids for metabolic purposes in muscles and adipose tissue.

Application

Lipoprotein Lipase from Burkholderia sp. has been used in the subcellular fractionation of mussel Mytilus galloprovincialis to identify the biomolecule attached to cytosolic fraction of okodaic acid. It has also been used to test its effect on serum amyloid A induced granulocyte colony-stimulated factor (G-CSF) expression in response to bacterial lipoprotein.LipoproteinLipase from Burkholderia sp. is suitable for the synthesis ofdiarylmethanols, a precursor for the synthesis of pharmaceutical compounds.

Lipoprotein lipase has been used in a study to assess the role of lipogenic enzymes in colorectal cancer. It has also been used in a study to investigate lipasemic activity of low molecular weight heparin in rats.

Biochem/physiol Actions

Lipoprotein Lipase (LPL) hydrolysis triacylglycerol moieties in chylomicron and low density lipoproteins. LPL attaches lipoprotein to the vessel wall and facilitates their uptake. Abnormalities in LPL is associated with Alzheimer′s disease, atherosclerosis, obesity and chylomicronaemia.

Due to its lipolytic activity, lipoprotein lipase was shown to effectively block the spread of hepatitis C virus into healthy cells.

Lipoprotein lipase belongs to the family of triglyceride lipases. It hydrolyses triglycerides in triglyceride-rich ApoB-containing lipoproteins.

Other Notes

One unit will release 1.0 nmole of p-nitrophenol per min at pH 7.2 at 37 °C using p-nitrophenyl butyrate as substrate.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)