Skip to Content
MilliporeSigma

Key Documents

View All Documentation

Skip To

C8868

Cholesterol Oxidase from microorganisms

lyophilized powder, ≥50 units/mg protein, recombinant, expressed in E. coli

Synonym(s):

Cholesterol: oxygen oxidoreductase

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Size/SKUAvailabilityPrice
100 units

Available to ship TODAYfromMILWAUKEE

$245.00
500 units

Available to ship TODAYfromMILWAUKEE

$827.00

About This Item

CAS Number:
9028-76-6
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-842-1
MDL number:
MFCD00130783
EC Number:
1.1.3.6 (BRENDA, IUBMB)
Specific activity:
≥50 units/mg protein
Recombinant:
expressed in E. coli

$245.00

Available to ship TODAYDetails

​
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

recombinant

expressed in E. coli

form

lyophilized powder

specific activity

≥50 units/mg protein

mol wt

64 kDa

composition

Protein, ≥15% biuret

storage temp.

−20°C

General description

Type II cholesterol oxidase containing FAD cofactor covalently linked to the enzyme.

Application

Cholesterol oxidase has been used in a study to demonstrate that microheterogeneity in hydrophobic-hydrophilic block copolymer systems can be exploited for immobilizing enzymes and to carry out enzymatic reactions. Cholesterol oxidase has also been used in a study that concluded that amphipaths that activate cholesterol might be useful in treating NPC disease.
Cholesterol oxidase is used to determine serum cholesterol.[1][2] The enzyme also finds application in the microanalysis of steroids in food samples and in distinguishing 3-ketosteroids from 3β-hydroxysteroids.[3] Transgenic plants expressing cholesterol oxidase are being investigated in the fight against the cotton boll weevil.[4] CHOD has also been used as a molecular probe to elucidate cellular membrane structures.[5][6]

Biochem/physiol Actions

Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This bifunctional enzyme oxidizes cholesterol to cholest-5-en-3-one in an FAD-requiring step, which is then isomerized to cholest-4-en-3-one with the release of H2O2.[7]

Physical form

Supplied as a lyophilzed powder containing sucrose

Other Notes

One unit will convert 1.0 μmole of cholesterol to 4-cholesten-3-one per min at pH 7.5 at 25 °C. Note: 4-cholesten-3-one may undergo isomerization.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
C1235C5421C4405
Cholesterol Oxidase from microorganisms lyophilized powder, ≥50 units/mg protein, recombinant, expressed in E. coli

Sigma-Aldrich

C8868

Cholesterol Oxidase from microorganisms

Cholesterol Oxidase microbial recombinant, lyophilized powder, ≥10 units/mg protein

Sigma-Aldrich

C1235

Cholesterol Oxidase microbial

Cholesterol Oxidase from microorganisms aqueous solution, ≥30 units/mg protein (biuret)

Sigma-Aldrich

C5421

Cholesterol Oxidase from microorganisms

Choline Oxidase from Arthrobacter globiformis lyophilized powder, 8-20 units/mg solid

Sigma-Aldrich

C4405

Choline Oxidase from Arthrobacter globiformis

specific activity

≥50 units/mg protein

specific activity

≥10 units/mg protein

specific activity

≥30 units/mg protein (biuret)

specific activity

8-20 units/mg solid

form

lyophilized powder

form

lyophilized powder

form

aqueous solution

form

lyophilized powder

recombinant

expressed in E. coli

recombinant

-

recombinant

-

recombinant

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−70°C

storage temp.

−20°C

mol wt

64 kDa

mol wt

55 kDa

mol wt

62 kDa

mol wt

71 kDa by SDS-PAGE, 83 kDa by gel filtration

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Still not finding the right product?

Explore all of our products under Cholesterol Oxidase from microorganisms

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Questions

Reviews

No rating value

Active Filters