5.09227

G 418 Sulfate, Sterile-Filtered Solution in PBS, Cell Culture-Tested

• Synonym(e): G 418 Sulfate, Sterile-Filtered Solution in PBS, Cell Culture-Tested, G418, G 418
• Empirische Formel (Hill-System): C₂₀H₄₀N₄O₁₀ · 2H₂SO₄
• CAS-Nummer: 108321-42-2
• Molekulargewicht: 692.71
• UNSPSC Code: 12352203
• MDL number: MFCD00058314

Eigenschaften

• assay: ≥98% (TLC)
• Quality Segment: 200
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles, protect from light
• storage temp.: 2-8°C
• InChI: 1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1
• InChI key: UHEPSJJJMTWUCP-NKCAIAFTSA-N

Beschreibung

General description

The optimal concentration of G418 for selection of resistance will vary according to the organism and/or cell type under investigation. In general, the concentration of active drug required for selection is as follows: Dictyostelium sp.: 10-100 µg/ml Plant cells: 10-100 µg/ml Yeast cells: 0.5-1.0 µg/ml Mammalian cells: 0.1-2.0 µg/ml

Packaging

Packaged under inert gas

Physical form

Sterile-filtered solution of Cat. No. 345810 supplied at 50 mg/ml in PBS, pH 7.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 1 year at -20°C.

Other Notes

Ethier, S.P., and Taback, E. 1993. Cancer Lett.74, 189.
Santerre, R.F., et al. 1991. Methods Mol. Biol.7, 245.
Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol.133, 46.
Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol.163, 8.
Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA79, 5166.
Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA79, 7356.
Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun.101, 1031.
Jimenez, A., and Davies, J. 1980. Nature287, 869.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• pictograms: GHS08
• signalword: Danger
• pcodes: P261 - P272 - P280 - P284 - P302 + P352 - P304 + P340 + P312
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 3
• flash_point_c: Not applicable
• flash_point_f: Not applicable
• hcodes: H317,H334