- A BODIPY-luminol chemiluminescent resonance energy-transfer (CRET) cassette for imaging of cellular superoxide.
A BODIPY-luminol chemiluminescent resonance energy-transfer (CRET) cassette for imaging of cellular superoxide.
Organic & biomolecular chemistry (2014-12-17)
S Bag, J-C Tseng, J Rochford
PMID25500943
ABSTRACT
Spectroscopic and in cellulo studies are here reported on the very first BODIPY-luminol chemiluminescent resonance energy-transfer (CRET) cassette where the luminol CL agent is covalently linked to the BODIPY energy-transfer acceptor in a molecular dyad. The efficiency of intramolecular CRET investigated for the BODIPY-luminol dyad was found to be 64% resulting in a dual emissive response. Successful in cellulo biochemiluminescence via CRET was achieved in PMA activated splenocytes.
MATERIALS
Product Number
Brand
Product Description
Boron, monofilament, 20m, diameter 0.1mm
Boron, monofilament, 100m, diameter 0.1mm
Boron, monofilament, 20m, diameter 0.2mm
Boron, monofilament, 10m, diameter 0.1mm
Boron, monofilament, 50m, diameter 0.1mm
Boron, monofilament, 50m, diameter 0.2mm
Boron, monofilament, 10m, diameter 0.2mm
Boron, monofilament, 5m, diameter 0.1mm
Boron, monofilament, 5m, diameter 0.2mm
Boron, monofilament, 200m, diameter 0.1mm