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  • Regulation of aldosterone biosynthesis by the Kir3.4 (KCNJ5) potassium channel.

Regulation of aldosterone biosynthesis by the Kir3.4 (KCNJ5) potassium channel.

Clinical and experimental pharmacology & physiology (2013-07-09)
Carolina Velarde-Miranda, Elise P Gomez-Sanchez, Celso E Gomez-Sanchez
ABSTRACT

The G-protein-activated inwardly rectifying potassium channel Kir3.4 is expressed in the zona glomerulosa cell membrane and transports potassium out of the cell.  Angiotensin II stimulation of aldosterone secretion is mediated, in part, by suppression of the transcription of KCNJ5, the gene coding for Kir3.4, and blocking channel activity. This results in membrane depolarization, mobilization of intracellular calcium, activation of the calcium-calmodulin pathway and increasing gene transcription of steroidogenic enzymes required for aldosterone secretion.  In 40-60% of aldosterone-producing adenomas there is a somatic mutation in the region of the KCNJ5 gene that codes for the selectivity filter that decreases potassium selectivity, allowing sodium to leak into the cells, thus depolarizing the membrane and initiating events that result in increased aldosterone synthesis.  The mechanism by which mutated KCNJ5 induces cell proliferation and adenoma formation remains unclear.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Potassium, chunks (in mineral oil), 98% trace metals basis
Pricing and availability is not currently available.
Sigma-Aldrich
Aldosterone, ≥95% (HPLC)
Pricing and availability is not currently available.
Supelco
Aldosterone solution, 100 μg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®
Pricing and availability is not currently available.