- Crystallization and preliminary X-ray analysis of a dye-linked D-lactate dehydrogenase from the aerobic hyperthermophilic archaeon Aeropyrum pernix.
Crystallization and preliminary X-ray analysis of a dye-linked D-lactate dehydrogenase from the aerobic hyperthermophilic archaeon Aeropyrum pernix.
Acta crystallographica. Section F, Structural biology and crystallization communications (2011-11-22)
Takenori Shibahara, Takenori Satomura, Ryushi Kawakami, Toshihisa Ohshima, Haruhiko Sakuraba
PMID22102248
ABSTRACT
A dye-linked D-lactate dehydrogenase from the aerobic hyperthermophilic archaeon Aeropyrum pernix was crystallized using the hanging-drop vapour-diffusion method with polyethylene glycol 8000 as the precipitant. The crystals belonged to the monoclinic space group P2(1), with unit-cell parameters a = 63.4, b = 119.4, c = 70.2 Å, β = 112.0°, and diffracted to 2.0 Å resolution on the BL26B1 beamline at SPring-8. The overall R(merge) was 4.5% and the completeness was 99.8%.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
D-Lactic Dehydrogenase from Lactobacillus leichmannii, lyophilized powder, 150-500 units/mg protein
Sigma-Aldrich
D-Lactic Dehydrogenase from Lactobacillus leichmannii, ammonium sulfate suspension, ≥250 units/mg protein (biuret)
Sigma-Aldrich
D-Lactic Dehydrogenase from Staphylococcus epidermidis, lyophilized powder, ≥80 units/mg solid