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  • Synthesis of chiral cyanohydrins by recombinant Escherichia coli cells in a micro-aqueous reaction system.

Synthesis of chiral cyanohydrins by recombinant Escherichia coli cells in a micro-aqueous reaction system.

Applied and environmental microbiology (2012-05-01)
Kathrin Emmi Scholz, Daniel Okrob, Benita Kopka, Alexander Grünberger, Martina Pohl, Karl-Erich Jaeger, Ulrich Krauss
PMID22544258
ABSTRACT

Synthesis of chiral cyanohydrins is performed in a monophasic micro-aqueous reaction system using whole recombinant Escherichia coli cells expressing the Arabidopsis thaliana hydroxynitrile lyase (AtHNL). Microscopy studies employing a fusion of AtHNL with a flavin-based fluorescent protein (FbFP) reveal that the cells remain intact in the reaction system.